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BioAim Scientific (Kanada)
The Bioaim Human Dkk-3 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Dkk-3 in human serum and plasma. An antibody specific for human Dkk-3 was coated on a 96-well plate. Standards and samples are added to the wells and any Dkk-3 present binds to the immobilized antibody. The wells are washed and biotinylated anti-human Dkk-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Dkk-3 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Apoptosis; embryonic development;
Human
dickkopf WNT signaling pathway inhibitor 3 (Dkk-3 ) Background: Members of the dickkopf-related protein family (Dkk-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. Dkk-3 and -4 also have one prokineticin domain. Dkk-1, -2, and -4 function as antagonists of canonical Wnt signaling by binding to LRP5/6, preventing interaction of LRP5/6 with Wnt-Frizzled complexes. Dkk-1, -2, and -4 also bind cell surface Kremen-1 or -2 and promote the internalization of LRP5/6. Antagonistic activity of Dkk-3 has not been demonstrated. Dkk proteins have distinct patterns of expression in adult and embryonic tissues and have a wide range of effects on tissue development and morphogenesis. The Dkk family also includes Soggy, which is homologous to Dkk-3 but not to the other family members.
REIC, RIG
< 1 pg/ml
2.7-2000 pg/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
Q9UBP4
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | < 1 pg/ml |
Specific Against | Human |
Antigen Name | dickkopf WNT signaling pathway inhibitor 3 |
Detection Range | 2.7-2000 pg/ml |
Alias | REIC, RIG |