Human Decorin ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human Decorin ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Decorin in human serum and plasma. An antibody specific for human Decorin was coated on a 96-well plate. Standards and samples are added to the wells and any Decorin present binds to the immobilized antibody. The wells are washed and biotinylated anti-human Decorin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Decorin present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis;

specific against

Human

detection target

Decorin (Decorin )
Background: Decorin is a proteoglycan that is on average 90 – 140 kDa. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS). Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. Decorin and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. Decorin promotes myoblast differentiation, supports angiogenesis, and inhibits tumor progression.

alias

CSCD, DSPG2, PG40, PGII, PGS2, SLRR1B

sensitivity

< 1 pg/ml

detection range

0.96-700 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P07585