Human CTACK ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human CTACK ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of CTACK in human serum and plasma. An antibody specific for human CTACK was coated on a 96-well plate. Standards and samples are added to the wells and any CTACK present binds to the immobilized antibody. The wells are washed and biotinylated anti-human CTACK antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of CTACK present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Inflammation;

specific against

Human

detection target

C-C motif chemokine ligand 27 (CTACK )
Background: CCL27, also known as Cutaneous T cell-attracting chemokine (CTACK), ALP, ILC and ESkine, is a CC chemokine discovered using expressed sequence tags (EST) in a homology search for novel chemokines. It is a small cytokine belonging to the CC chemokine family also known under the names IL-11 R-alpha-locus chemokine (ILC), Skinkine, ESkine and Cutaneous T-cell-attracting chemokine (CTACK). It is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediated inflammation of the skin. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotactic effects by binding to the chemokine receptor CCR10. Mature human and mouse CCL27 are 84% similar. Mouse CCL27 shares 37% and 35% amino acid identity with mouse MIP-3 beta and mouse TECK, respectively.

alias

ALP, CTACK, CTAK, ESKINE, ILC, PESKY, SCYA27

sensitivity

8 pg/ml

detection range

13.7-10000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q9Y4X3