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Human CT1 ELISA Kit

Product Code: BAS-3010050

Verfügbarkeit: Auf Lager

Human CT1 ELISA Kit

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Beschreibung

Details

Human CT1 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human CT1 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of CT1 in human serum and plasma. An antibody specific for human CT1 was coated on a 96-well plate. Standards and samples are added to the wells and any CT1 present binds to the immobilized antibody. The wells are washed and biotinylated anti-human CT1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of CT1 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Inflammation;

specific against

Human

detection target

cardiotrophin 1 (CT1 )
Background: Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit.

alias

CT-1, CT1

sensitivity

10 pg/ml

detection range

16.5-12000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q16619

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller BioAim Scientific
Herstellerland Kanada
Sensitivity 10 pg/ml
Specific Against Human
Antigen Name cardiotrophin 1 
Detection Range 16.5-12000 pg/ml
Alias CT-1, CT1
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