BioAim Scientific (Kanada)
The Bioaim Human CRP ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of CRP in human serum and plasma. An antibody specific for human CRP was coated on a 96-well plate. Standards and samples are added to the wells and any CRP present binds to the immobilized antibody. The wells are washed and biotinylated anti-human CRP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of CRP present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Inflammation;
C-reactive protein, pentraxin-related(CRP ) Background: C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. It induces the phagocytosis of bacteria and apoptotic cells and regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.
Serum, plasma, Cell culture supernatant
|Antigen Name||C-reactive protein, pentraxin-related|
|Detection Range||34.3-25000 pg/ml|