For the quantitative determination of Human Complement Component C2 concentration in serum, or cell culture supernates.
The average concentration of C02 in 10 normal human serum is 47.86 +/- 14.60 µg/mL ranging from 21.43 to 67.86 µg/mL. HepG2 cells (0.2 x 10E5 cells/mL) were cultured 3 days. An aliquot of the cell culture supernate was removed, assayed for levels of natural CO2, and measured 252.51 ng/mL.
This assay recognizes both recombinant and natural Human CO2. Human C1S and C5a were prepared at 300 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
C2Antigene: 10154-H08Hcapture antibody type: rabbit mAbdetection antibody type
CEACAM6 / CD66c
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Antigen Name||Complement Component C2|
|Detection Range||234.38-15000 pg/ml|
|Alias||Complement Component C2|