Human Coagulation Factor IX / FIX / F9 ELISA Pair Set

Brand

Sino Biological

short description

The Human Coagulation Factor IX / FIX / F9 ELISA Pair Set is for the quantitative determination of Human Coagulation Factor IX / FIX / F9. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human Coagulation Factor IX / FIX / F9 coated on a 96-well plate. Standards and samples are added to the wells, and any Human Coagulation Factor IX / FIX / F9 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human Coagulation Factor IX / FIX / F9 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human Coagulation Factor IX / FIX / F9 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

spezie

Human

detection target

F9
Standard (antigene): Each vial contains 147 ng of recombinant Human Coagulation Factor IX / FIX / F9. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 7000 pg/mL is recommended.
capture antibody type: 1 mg/mL of rabbit anti-Human Coagulation Factor IX / FIX / F9 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 µg/mL in PBS before coating. (Catalog: # 11503-R044)
detection antibody type: 0.5 mg/mL of rabbit anti-Human Coagulation Factor IX / FIX / F9 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.8 µg/mL in detection antibody dilution buffer before use. (Catalog: # 11503-R014)
Lable: HRP

sensitivity

The minimum detectable dose of Human Coagulation Factor IX / FIX / F9 was determined to be approximately 109.38 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

109.38 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.