Human Coagulation Factor III ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human Coagulation factor III ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Coagulation factor III in Human serum, plasma and cell culture media. An antibody specific for Human Coagulation factor III was coated on a 96-well plate. Standards and samples are added to the wells and any Coagulation factor III present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human Coagulation factor III antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Coagulation factor III present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Coagulation ;

specific against

Human

detection target

Coagulation factor III(Coagulation factor III)
Background: Coagulation Factor III/Tissue Factor (TF), also known as Thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of coagulation factor VII, which is synthesized in the liver and circulated in the plasma. TF is the cell surface receptor for the serine protease factor VIIa. The best known function of tissue factor is its role in blood coagulation. The complex of TF with factor VIIa activates factor IX and catalyzes the conversion of the inactive protease factor X into the active protease factor Xa. Together with factor VIIa, tissue factor forms the tissue factor or extrinsic pathway of coagulation. This is opposed to the intrinsic (amplification) pathway which involves both activated factor IX and factor VIII.

alias

CD142, TF, TFA

sensitivity

2 pg/ml

detection range

6.1-1500 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P13726