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Human CLEC4A / CLECSF6 / DCIR ELISA Pair Set

Product Code: SEK11476

Verfügbarkeit: Auf Lager

Human CLEC4A / CLECSF6 / DCIR ELISA Pair Set

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Beschreibung

Details

Human CLEC4A / CLECSF6 / DCIR ELISA Pair Set

Brand

Sino Biological

short description

The human DCIR / CLEC4A / CLECSF6 ELISA Pair Set is for the quantitative determinationof human DCIR / CLEC4A / CLECSF6. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for DCIR / CLEC4A / CLECSF6 coated on a 96-well plate. Standards and samples are added to the wells, and any DCIR / CLEC4A / CLECSF6 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-DCIR / CLEC4A / CLECSF6 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of DCIR / CLEC4A / CLECSF6 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

spezie

Human

detection target

CLEC4A
Standard (antigene): Each vial contains 45 ng of recombinant CLEC4A. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 600 pg/mL is recommended.
capture antibody type: 0.2 mg/mL of mouse anti-CLEC4A monoclonal antibody, Dilute to a working concentration of 2.0 µg/mL in CBS before coating. (Catalog: # 11476-MM04)
detection antibody type: 0.5 mg/mL mouse anti-CLEC4A monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 µg/mL in detection antibody dilution buffer before use. (Catalog: # 11476-MM02)
Lable: HRP

sensitivity

The minimum detectable dose of Human DCIR / CLEC4A / CLECSF6 was determined to be approximately 9.375 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

9.375 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.

Weitere Informationen

Weitere Informationen

Hersteller Sino Biological
Herstellerland China
Sensitivity The minimum detectable dose of Human DCIR / CLEC4A / CLECSF6 was determined to be approximately 9.375 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Specific Against Human
Antigen Name CLEC4A
Detection Range 9.375 pg/ml
Zertifikat ISO9001-2008
Alias CLEC4A / CLECSF6 / DCIR
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