The Human CHL-1 ELISA Pair Set is for the quantitative determination of Human CHL-1. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CHL-1 coated on a 96-well plate. Standards and samples are added to the wells, and any CHL-1 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-CHL-1 monoclonal antibody is then added, producing an antibody-antigen-antibody ﾓsandwichﾔ. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CHL-1 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
CHL1Standard (antigene): Each vial contains 80 ng of recombinant CHL1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended. capture antibody type: 0.2 mg/mL of mouse anti-CHL1 monoclonal antibody, Dilute to a working concentration of 2 µg/mL in CBS before coating. (Catalog: # 10143-MM05)detection antibody type: 0.5 mg/mL mouse anti-CHL1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 µg/mL in detection antibody dilution buffer before use. (Catalog: # 10143-MM01)Lable: HRP
The minimum detectable dose of Human CHL-1 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of Human CHL-1 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|Detection Range||31.25 pg/ml|