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Human CEACAM6 / CD66c ELISA Pair Set - SEK10823

Product Code: SEK10823

Verfügbarkeit: Auf Lager

Human CEACAM6 / CD66c ELISA Pair Set - SEK10823

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Beschreibung

Details

Human CEACAM6 / CD66c ELISA Pair Set

Brand

Sino Biological

short description

The human CEACAM6 / CD66c ELISA Pair Set is for the quantitative determination of human CEACAM6 / CD66c. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CEACAM6 / CD66c coated on a 96-well plate. Standards and samples are added to the wells, and any CEACAM6 / CD66c present binds to the immobilized antibody. The wells are washed and a biotinylated rabbit anti-CEACAM6 / CD66c polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". To produces color in proportion to the amount of CEACAM6 / CD66c present in the sample strepavidin-HRP and TMB substrate solution are loaded. The absorbances of the microwell are read at 450 nm.

spezie

Human

detection target

CEACAM6
Standard (antigene): Each vial contains 88 ng of recombinant CEACAM6. Reconstitute standard powder with 1 mL detection antibody dilution buffer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
capture antibody type: 1 mg/mL of mouse anti-CEACAM6 monoclonal antibody. Dilute to a working concentration of 2 µg/mL in PBS before coating.
detection antibody type: 0.4 mg/mL of biotinylated rabbit anti-CEACAM6 polyclonal antibody. Dilute to working concentration of 0.5 µg/mL in detection antibody dilution buffer before use.
Lable: Biotin

sensitivity

The minimum detectable dose of human CEACAM6 / CD66c was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

15.6 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles. Streptavidin-HRP: Store at 4°C and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.

Weitere Informationen

Weitere Informationen

Hersteller Sino Biological
Herstellerland China
Sensitivity The minimum detectable dose of human CEACAM6 / CD66c was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Specific Against Human
Antigen Name CEACAM6
Detection Range 15.6 pg/ml
Zertifikat ISO9001-2008
Alias CEACAM6 / CD66c
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