Profile Informationen
Login Daten
oder Einloggen
Sino Biological
The human CEACAM6 / CD66c ELISA Pair Set is for the quantitative determination of human CEACAM6 / CD66c. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CEACAM6 / CD66c coated on a 96-well plate. Standards and samples are added to the wells, and any CEACAM6 / CD66c present binds to the immobilized antibody. The wells are washed and a biotinylated rabbit anti-CEACAM6 / CD66c polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". To produces color in proportion to the amount of CEACAM6 / CD66c present in the sample strepavidin-HRP and TMB substrate solution are loaded. The absorbances of the microwell are read at 450 nm.
Human
CEACAM6Standard (antigene): Each vial contains 88 ng of recombinant CEACAM6. Reconstitute standard powder with 1 mL detection antibody dilution buffer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended. capture antibody type: 1 mg/mL of mouse anti-CEACAM6 monoclonal antibody. Dilute to a working concentration of 2 µg/mL in PBS before coating.detection antibody type: 0.4 mg/mL of biotinylated rabbit anti-CEACAM6 polyclonal antibody. Dilute to working concentration of 0.5 µg/mL in detection antibody dilution buffer before use.Lable: Biotin
The minimum detectable dose of human CEACAM6 / CD66c was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
15.6 pg/ml
ISO9001-2008
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles. Streptavidin-HRP: Store at 4°C and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
Hersteller | Sino Biological |
---|---|
Herstellerland | China |
Sensitivity | The minimum detectable dose of human CEACAM6 / CD66c was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. |
Specific Against | Human |
Antigen Name | CEACAM6 |
Detection Range | 15.6 pg/ml |
Zertifikat | ISO9001-2008 |
Alias | CEACAM6 / CD66c |