Human CD93/C1qR PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human CD93 in cell culture supernates, serum, plasma( heparin, EDTA), saliva and urine.

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human CD93
Protein name: Complement component C1q receptor
Gene Full Name: CD93 molecule
abbreviation: CD93
Background: CD93 (Cluster of Differentiation 93) is a protein that in humans is encoded by the CD93 gene. CD93 belongs to the Group XIV C-Type lectin family and is mapped to 20p11.21. The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton. It has also showed that this receptor is a positive marker for all bone marrow-repopulating stem cells because it is expressed on both CD34-negative and CD34-positive stem cells from umbilical cord blood and adult bone marrow. In addition, highly purified lineage-negative cells with the CD93 protein not only have bone marrow-repopulating capacity but also can differentiate into human hepatocytes in vivo.
ID:Q9NPY3
ID:22918

alias

C1q receptor 1|C1q/MBL/SPA receptor|C1qR|C1qR(p)|C1qr1|C1QR1_HUMAN|C1qRp|CD93|CD93 antigen|CD93 molecule|CDw93|Complement component 1 q subcomponent receptor 1|Complement component C1q receptor|dJ737E23.1|ECSM3|Matrix remodeling associated protein 4|Matrix remodelling associated 4|Matrix-remodeling-associated protein 4|MXRA4

Origin

Nativ and recombinant human CD93

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, A24-K580

sensitivity

<10pg/ml

detection range

78pg/ml-5000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|634|1493|2436|572|1620|2864
Standard deviation|33.6|92.6|107.2|38.9|121.5|160.4
CV(%)|5.3|6.2|4.4|6.8|7.5|5.6

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human CD93 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD93 has been precoated onto 96-well plates. Standards(NSO, A24-K580) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD93 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD93 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml, 78pg/ml human CD93 standard solutions into the pre-coated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA), saliva or urine to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each human CD93 standard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|78|156|313|625|1250|2500|5000 O.D.|0.007|0.203|0.377|0.702|1.104|1.456|1.814|2.028

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human CD93 antibody|1
ST0000-10|Lyophilized recombinant human CD93 standard|10ng/tube�2
AR1107|Biotinylated anti- human CD93 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4