For the quantitative determination of Human CD86 / B7-2 concentration in serum.
10 normal human serum were tested for natural B7Ǆ, the average concentration of B7Ǆ in 6 normal human serum is 654.21 +/- 468.83 pg/mL ranging from 132.57 to 1200.00 pg/mL. Four samples measured less than the lowest standard, 6.25 pg/mL.
This assay recognizes both recombinant and natural Human B7-2. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
CD86Antigene: 10699-H08Hcapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||6.25-400 pg/ml|
|Alias||CD86 / B7-2|