Human CD48 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human CD48 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human CD48
Protein name: CD48 antigen
Gene Full Name: CD48 molecule
abbreviation: CD48
Background: CD48 antigen (Cluster of Differentiation 48), also known as BLAST-1 or SLAMF2, is a protein that in humans is encoded by the CD48 gene. CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily (IgSF) which includes SLAM (signaling lymphocyte activation molecules) proteins, such as CD84, CD150, CD229 and CD244. It is mapped to 1q23.3. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor.
ID:P09326
ID:962

alias

Antigen CD48 | BCM 1 surface antigen | Bcm-1 | BCM1 surface antigen | BCM1, BLAST1 | BLAST | CD48 antigen | CD48 molecule | CD48 protein | Leucocyte antigen MEM 102 | Leukocyte antigen MEM-102 | MEM102 | SLAMF2 | TCT.1 | P09326

Origin

Nativ and recombinant human CD48

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, Q27-S220

sensitivity

<12pg/ml

detection range

62.5pg/ml-4000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|269|1347|2218|302|1588|2436
Standard deviation|18.29|96.98|141.95|25.67|136.57|224.11
CV(%)|6.8|7.2|6.4|8.5|8.6|9.2

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human CD48 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD48 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q27-S220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD48 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD48 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human CD48 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human CD48 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.007|0.054|0.122|0.216|0.472|0.877|1.610|2.033

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human CD48 antibody|1
ST0000-10|Lyophilized recombinant human CD48 standard|10ng/tube�2
AR1107|Biotinylated anti- human CD48 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4