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Human CD47 PicoKine ELISA Kit

Product Code: BOS-EK1421

Verfügbarkeit: Auf Lager

Human CD47 PicoKine ELISA Kit

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Beschreibung

Details

Human CD47 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human CD47 in cell culture supernates and serum.

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human CD47
Protein name: Leukocyte surface antigen CD47
Gene Full Name: CD47 molecule
abbreviation: CD47
Background: CD47 (Cluster of Differentiation 47), also known as integrin associated protein (IAP), is a transmembrane protein that in humans is encoded by the CD47 gene. It belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. Also CD47 is ubiquitously expressed in human cells and has found to be overexpressed in many different tumor cells.
UniProd ID:Q08722
NCBI ID:961

alias

Antigen identified by monoclonal antibody 1D8|Antigenic surface determinant protein OA3|CD 47|CD47|CD47 antigen (Rh-related antigen, integrin-associated signal transducer)|CD47 antigen|CD47 glycoprotein|CD47 molecule|CD47_HUMAN|IAP|Integrin Associated Protein| Integrin associated signal transducer|Integrin-associated protein|Leukocyte surface antigen CD47|MER6|OA 3|OA3|OTTHUMP00000041152| OTTHUMP00000041153|Protein MER6|Rh related antigen

Origin

Nativ and recombinant human CD47

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO,Q19-P139

sensitivity

<10pg/ml

detection range

125pg/ml-8000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|347|1200|5260|450|1823|5896
Standard deviation|17|66|326.12|28.8|123.96|442.2
CV(%)|4.9|5.5|6.2|6.4|6.8|7.5

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human CD47 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD47 has been precoated onto 96-well plates. Standards (NSO, Q19 - P139) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD47 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD47 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml human CD47 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates or serum to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human CD47 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|125|250|500|1000|2000|4000|8000 O.D.|0.004|0.102|0.175|0.333|0.559|1.021|1.672|2.054

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human CD47 antibody|1
ST0000-10|Lyophilized recombinant human CD47 standard|10ng/tube�2
AR1107|Biotinylated anti- human CD47 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller Boster
Herstellerland USA
Sensitivity <10pg/ml
Specific Against Human
Antigen Name Leukocyte surface antigen CD47 (CD47)
Detection Range 125pg/ml-8000pg/ml
Alias Antigen identified by monoclonal antibody 1D8, Antigenic surface determinant protein OA3, CD 47, CD47, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 antigen, CD47 glycoprotein, CD47 molecule, CD47_HUMAN, IAP, Integrin Asso
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Datasheet