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Human CD40 / TNFRSF5 ELISA Pair Set

Product Code: SEK10774

Verfügbarkeit: Auf Lager

Human CD40 / TNFRSF5 ELISA Pair Set

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Beschreibung

Details

Human CD40 / TNFRSF5 ELISA Pair Set

Brand

Sino Biological

short description

The human CD40 / TNFRSF5 ELISA Pair Set is for the quantitative determinationof human CD40 . This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD40 / TNFRSF5 coated on a 96-well plate. Standardsand samples are added to the wells, and any CD40 / TNFRSF5present binds to the immobilized antibody. The wells are washed and ahorseradish peroxidase conjugated rabbit anti-CD40 / TNFRSF5 monoclonal antibody is then added, producing anantibody-antigen-antibody "sandwich". The wells are again washed and TMBsubstrate solution is loaded, which produces color in proportion to the amountof CD40 / TNFRSF5 present in the sample. To end theenzyme reaction, the stop solution is added and absorbances of the microwellare read at 450 nm.

spezie

Human

detection target

CD40
Standard (antigene): Each vial contains 20 ng of recombinant CD40. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 250 pg/mL is recommended
capture antibody type: 1.0 mg/mL of mouse anti-CD40 monoclonal antibody. Dilute to a working concentration of 2.0 µg/mL in CBS before coating (Catalog: # 10774-MM11)
detection antibody type: 0.5 mg/mL rabbit anti-CD40 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 µg/mL in detection antibody dilution buffer before use. (Catalog: # 10774-R004)
Lable: HRP

sensitivity

The minimum detectable dose of human CD40 / TNFRSF5 was determined to be approximately 3.9 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard

Detection limitation

3.9 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.

Weitere Informationen

Weitere Informationen

Hersteller Sino Biological
Herstellerland China
Sensitivity The minimum detectable dose of human CD40 / TNFRSF5 was determined to be approximately 3.9 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard
Specific Against Human
Antigen Name CD40
Detection Range 3.9 pg/ml
Zertifikat ISO9001-2008
Alias CD40 / TNFRSF5
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