The human CD40 / TNFRSF5 ELISA Pair Set is for the quantitative determinationof human CD40 . This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD40 / TNFRSF5 coated on a 96-well plate. Standardsand samples are added to the wells, and any CD40 / TNFRSF5present binds to the immobilized antibody. The wells are washed and ahorseradish peroxidase conjugated rabbit anti-CD40 / TNFRSF5 monoclonal antibody is then added, producing anantibody-antigen-antibody "sandwich". The wells are again washed and TMBsubstrate solution is loaded, which produces color in proportion to the amountof CD40 / TNFRSF5 present in the sample. To end theenzyme reaction, the stop solution is added and absorbances of the microwellare read at 450 nm.
CD40Standard (antigene): Each vial contains 20 ng of recombinant CD40. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 250 pg/mL is recommended capture antibody type: 1.0 mg/mL of mouse anti-CD40 monoclonal antibody. Dilute to a working concentration of 2.0 µg/mL in CBS before coating (Catalog: # 10774-MM11)detection antibody type: 0.5 mg/mL rabbit anti-CD40 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 µg/mL in detection antibody dilution buffer before use. (Catalog: # 10774-R004)Lable: HRP
The minimum detectable dose of human CD40 / TNFRSF5 was determined to be approximately 3.9 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of human CD40 / TNFRSF5 was determined to be approximately 3.9 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard|
|Detection Range||3.9 pg/ml|
|Alias||CD40 / TNFRSF5|