Human CD40 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human CD40 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of CD40 in human serum and plasma. An antibody specific for human CD40 was coated on a 96-well plate. Standards and samples are added to the wells and any CD40 present binds to the immobilized antibody. The wells are washed and biotinylated anti-human CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of CD40 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Hematologic Cancer;

specific against

Human

detection target

CD40 (CD40 )
Background: CD40 is a co-stimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. CD40 is constitutively expressed by antigen presenting cells, including dendritic cells, B cells and macrophages. It can also be expressed by endothelial cells, smooth muscle cells, fibroblasts and epithelial cells. Consistent with its widespread expression on normal cells, CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin’s and Hodgkin’s lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary. CD40 is also expressed on B cell precursors in the bone marrow, and there is some evidence that CD40-CD154 interactions may play a role in the control of B cell haematopoiesis.

alias

Bp50, CDW40, TNFRSF5, p50

sensitivity

25 pg/ml

detection range

54.9-40000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P25942