Human CD320/8D6A PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human CD320 in cell culture supernates, serum, plasma(heparin, EDTA) and urine.

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human CD320
Protein name: CD320 antigen
Gene Full Name: CD320 molecule
abbreviation: CD320
Background: CD320(cluster of differentiation 320), also known as 8D6A or TCBLR, is a human gene. It is mapped to chromosome 19p13.2. This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin(vitamin B12), and the expression of CD320 enhanced B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. It has been found that antagonists of the CD320 signaling pathway may counter the growth of follicular lymphomas or other tumors that metastasize to lymphoid follicles. CD320 is also a specific receptor for uptake of TCN2-bound cobalamin.
ID:Q9NPF0
ID:51293

alias

8D6|8D6 antigen|8D6A|CD320|CD320 antigen|CD320_HUMAN|FDC-signaling molecule 8D6|FDC-SM-8D6|TCblR|Transcobalamin receptor

Origin

Nativ and recombinant human CD320

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, S36-Y229

sensitivity

<10pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|1.1|3.2|6.3|1.3|3.4|5.9
Standard deviation|0.042|0.14|0.31|0.08|0.194|0.313
CV(%)|3.8|4.5|4.9|6.2|5.7|5.3

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human CD320 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD320 has been precoated onto 96-well plates. Standards(NSO, S36-Y229) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD320 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD320 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human CD320 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human CD320 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.023|0.154|0.235|0.399|0.776|1.301|1.790|2.021

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human CD320 antibody|1
ST0000-10|Lyophilized recombinant human CD320 standard|10ng/tube�2
AR1107|Biotinylated anti- human CD320 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4