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Human CD32/FCGR2b/c PicoKine ELISA Kit

Product Code: BOS-EK1312

Verfügbarkeit: Auf Lager

Human CD32/FCGR2b/c PicoKine ELISA Kit

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Beschreibung

Details

Human CD32/FCGR2b/c PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human CD32 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human CD32
Protein name: Low affinity immunoglobulin gamma Fc region receptor II-b
Gene Full Name: Fc fragment of IgG, low affinity IIb, receptor (CD32)
abbreviation: FCGR2B
Background: CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.
UniProd ID:P31994&P31995
NCBI ID:2213&9103

alias

CD 32|CD32|CD32 antigen|CD32A|CD32B|CD32C|CDw32|Fc fragment of IgG low affinity II receptor for (CD32)|Fc fragment of IgG low affinity IIa receptor|Fc fragment of IgG low affinity IIa receptor for (CD32)|Fc fragment of IgG low affinity IIb receptor|Fc fragment of IgG low affinity IIb receptor for (CD32)|Fc fragment of IgG low affinity IIc receptor|Fc fragment of IgG low affinity IIc receptor for (CD32)|Fc gamma receptor IIB|Fc gamma RII a|Fc gamma RII b|Fc gamma RII c|Fc gamma RIIa|Fc gamma RIIb |Fc gamma RIIc|Fc receptor, IgG, low affinity IIb|Fc-gamma RII-a|Fc-gamma RII-b|Fc-gamma RII-c|Fc-gamma-RIIa|FC-gamma-RIIB|Fc-GAMMA-RIIC|Fc[g]RII|FCG 2|FCG2|FCG2A_HUMAN|FcgammaRIIC|FCGR 2|FcGR|FCGR2|FCGR2A|FCGR2A1|FCGR2B|FCGR2C|Fcgr3|Fcgr3a|FcgRII|Fcr-2|Fcr-3|FcRII a|FCRII|FcRII b|FcRII c|FcRII-a|FcRIIC|IGFR 2|IGFR2|IgG Fc receptor II a|IgG Fc receptor II b|IgG Fc receptor II beta|IgG Fc receptor II c|IgG Fc receptor II-a|IgG Fc receptor II-b|IgG Fc receptor II-c|Immunoglobulin G Fc receptor II|Low affinity|immunoglobulin gamma Fc region receptor II a|Low affinity immunoglobulin gamma Fc region receptor II|Low affinity immunoglobulin gamma Fc region receptor II b|Low affinity immunoglobulin gamma Fc region receptor II c|Low affinity immunoglobulin gamma Fc region receptor II-a|Low affinity immunoglobulin gamma Fc region receptor II-b|Low affinity immunoglobulin gamma Fc region receptor II-c|Ly-17|Ly-m20|LyM-1|Lymphocyte antigen 17|MGC23887|MGC30032

Origin

Nativ and recombinant human CD32

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, A46-P217

sensitivity

<10pg/ml

detection range

46.9pg/ml-3000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|147|890|1865|168|904|2162
Standard deviation|6.62|32.93|80.2|9.75|41.6|112.42
CV(%)|4.5|3.7|4.3|5.8|4.6|5.2

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human CD32 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD32 has been precoated onto 96-well plates. Standards(NSO, A46-P217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD32 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD32 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 3000pg/ml, 1500pg/ml, 750pg/ml, 375pg/ml, 187.5pg/ml, 93.8pg/ml, 46.9pg/ml human CD32 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. We recommend that each human CD32 standard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|46.9|93.8|187.5|375|750|1500|3000 O.D.|0.007|0.124|0.199|0.255|0.541|0.813|1.408|2.266

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human CD32 antibody|1
ST0000-10|Lyophilized recombinant human CD32 standard|10ng/tube�2
AR1107|Biotinylated anti- human CD32 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller Boster
Herstellerland USA
Sensitivity <10pg/ml
Specific Against Human
Antigen Name Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B)
Detection Range 46.9pg/ml-3000pg/ml
Alias CD 32, CD32, CD32 antigen, CD32A, CD32B, CD32C, CDw32, Fc fragment of IgG low affinity II receptor for (CD32), Fc fragment of IgG low affinity IIa receptor, Fc fragment of IgG low affinity IIa receptor for (CD32), Fc fragment of IgG low affinity IIb recep
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Datasheet