For the quantitative determination of Human CD30 / TNFRSF8 concentration in cell lysate.
The concentration of Human TNFRSF8 detected in a Jurkat cell lysate is 34.9ng/mL.The Jurkat cell lysate' total protein concentration was about 6mg/mL.
This assay recognizes both recombinant and natural Human TNFRSF8. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
TNFRSF8Antigene: 10777-H08Hcapture antibody type: mouse mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||23.44-1500 pg/ml|
|Alias||CD30 / TNFRSF8|