Human CD30 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human CD30 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of CD30 in Human serum, plasma and cell culture media. An antibody specific for Human CD30 was coated on a 96-well plate. Standards and samples are added to the wells and any CD30 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human CD30 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of CD30 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Apoptosis;

specific against

Human

detection target

TNF receptor superfamily member 8 (CD30)
Background: CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. It is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. CD30 is associated with anaplastic large cell lymphoma. It is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors.

alias

CD30, D1S166E, Ki-1

sensitivity

20 pg/ml

detection range

20.5-5000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P28908