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Human CD26/DPP4 PicoKine ELISA Kit

Product Code: BOS-EK0696

Verfügbarkeit: Auf Lager

Human CD26/DPP4 PicoKine ELISA Kit

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Beschreibung

Details

Human CD26/DPP4 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human CD26 in cell culture supernates, serum, plasma(heparin, EDTA), saliva and urine.

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human CD26
Protein name: Dipeptidyl peptidase 4
Gene Full Name: dipeptidyl-peptidase 4
abbreviation: DPP4
Background: Dipeptidyl peptidase-4(DPP4), also known as adenosine deaminase complexing protein 2 or CD26(cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. By fluorescence in situ hybridization, the CD26 gene was mapped to 2q24.3. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours. CD26/ DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.
UniProd ID:P27487
NCBI ID:1803

alias

ADABP|ADCP-2|ADCP2|Adenosine deaminase complexing protein 2|CD26|Dipeptidyl peptidase 4|Dipeptidyl peptidase 4 soluble form|Dipeptidyl peptidase IV|Dipeptidyl peptidase IV membrane form|Dipeptidyl peptidase IV soluble form|Dipeptidylpeptidase 4|DPP 4|DPP IV|DPP4|DPP4_HUMAN|DPPIV|T-cell activation antigen CD26|TP103

Origin

Nativ and recombinant human CD26

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, D34-P766

sensitivity

<10pg/ml

detection range

312pg/ml-20000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|1.58|7.23|12.07|1.66|7.59|12.83
Standard deviation|0.098|0.513|0.881|0.126|0.645|1.052
CV(%)|6.2|7.1|7.3|7.6|8.5|8.2

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human CD26 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD26 has been precoated onto 96-well plates. Standards(NSO, D34-P766) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD26 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD26 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human CD26 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA) saliva or urine to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human CD26 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|312|625|1250|2500|5000|10000|20000 O.D.|0.002|0.082|0.166|0.297|0.506|1.007|1.630|2.311

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human CD26 antibody|1
ST0000-20|Lyophilized recombinant human CD26 standard|20ng/tubex2
AR1107|Biotinylated anti- human CD26 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller Boster
Herstellerland USA
Sensitivity <10pg/ml
Specific Against Human
Antigen Name Dipeptidyl peptidase 4 (DPP4)
Detection Range 312pg/ml-20000pg/ml
Alias ADABP, ADCP-2, ADCP2, Adenosine deaminase complexing protein 2, CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 soluble form, Dipeptidyl peptidase IV, Dipeptidyl peptidase IV membrane form, Dipeptidyl peptidase IV soluble form, Dipeptidylpeptidase 4,
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Datasheet