Human CD25/IL-2sR alpha PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human CD25 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human CD25
Protein name: Interleukin-2 receptor subunit alpha
Gene Full Name: interleukin 2 receptor, alpha
abbreviation: IL2RA
Background: CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
ID:P01589
ID:3559

alias

CD122|CD122 antigen|CD132|CD132 antigen|CD25|CD25 antigen|Cytokine receptor common gamma chain|Gamma C|High affinity IL 2 receptor subunit beta|IDDM10|IL 2 RA|IL 2 receptor|IL 2 receptor alpha subunit|IL 2R gamma chain|IL2 RA|IL2R|IL2RA|IL2RB|IL2RG|IMD4|interleukin 2 receptor alpha|Interleukin 2 receptor alpha chain|interleukin 2 receptor beta|Interleukin 2 receptor beta chain|interleukin 2 receptor gamma|Interleukin 2 receptor gamma (severe combined immunodeficiency)|Interleukin 2 receptor gamma chain|p55|p64|P70 75|p75|SCIDX|SCIDX1|TAC antigen|TCGFR|XSCID

Origin

Nativ and recombinant human CD25

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO,E22-C213

sensitivity

<5pg/ml

detection range

78pg/ml-5000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|0.22|0.63|2.53|0.25|0.71|2.36
Standard deviation|0.014|0.041|0.132|0.020|0.052|0.191
CV(%)|6.3|6.5|5.2|8|7.3|8.1

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human CD25 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD25 has been precoated onto 96-well plates. Standards (NSO,E22-C213) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD25 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD25 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml, 78pg/ml human CD25 standard solutions into the pre-coated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each human CD25 standard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|78|156|313|625|1250|2500|5000 O.D.|0.016|0.251|0.435|0.770|1.108|1.479|1.853|2.049

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human CD25 antibody|1
ST0000-10|Lyophilized recombinant human CD25 standard|10ng/tubex2
AR1107|Biotinylated anti- human CD25 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4