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BioAim Scientific (Kanada)
The Bioaim Human CD244 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of CD244 in Human serum, plasma and cell culture media. An antibody specific for Human CD244 was coated on a 96-well plate. Standards and samples are added to the wells and any CD244 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human CD244 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of CD244 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Immune response;
Human
CD244 molecule(CD244) Background: 2B4, also known as CD244 and SLAMF4, is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family. SLAM family proteins have an extracellular domain (ECD) with two or four Ig-like domains and at least two cytoplasmic immunoreceptor tyrosine-based switch motifs (ITSMs). 2B4 interacts with CD48, while other SLAM family proteins interact homophilically. Three additional splice variants of human 2B4 have deletions of the short region between the Ig-like domains, the second Ig-like domain, or a portion of the cytoplasmic tail.
2B4, NAIL, NKR2B4, Nmrk, SLAMF4
25 pg/ml
0.049-12 ng/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
Q9BZW8
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | 25 pg/ml |
Specific Against | Human |
Antigen Name | CD244 molecule |
Detection Range | 0.049-12 ng/ml |
Alias | 2B4, NAIL, NKR2B4, Nmrk, SLAMF4 |