Human CD23 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human CD23 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of CD23 in Human serum, plasma and cell culture media. An antibody specific for Human CD23 was coated on a 96-well plate. Standards and samples are added to the wells and any CD23 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human CD23 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of CD23 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Immune response;

specific against

Human

detection target

Fc fragment of IgE receptor II (CD23)
Background: CD23, also known as Fc epsilon RII, or FcεRII, is the “low-affinity” receptor for IgE. Unlike many of the antibody receptors, CD23 is a C-type lectin. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. CD23 is known to have a role of transportation in antibody feedback regulation. The antigen is then transferred from CD23+ B cells to CD11c+ antigen presenting cells. The CD11c+ cells in turn present the antigen to CD4+ T cells, which can lead to an enhanced antibody response.

alias

BLAST-2, CD23, CD23A, CLEC4J, FCE2, IGEBF

sensitivity

2 pg/ml

detection range

4.9-1200 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P06734