Human CD21/CR2 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human CD21 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human CD21
Protein name: Complement receptor type 2
Gene Full Name: complement component (3d/Epstein Barr virus) receptor 2
abbreviation: CR2
Background: Complement receptor type 2(CR2), also known as C3DR or CD21, is a protein that in humans is encoded by the CR2 gene. It is mapped to 1q32.2. CR2 is involved in the complement system. It binds to iC3b(inactive derivative of C3b), C3dg, or C3d. CR2 is the membrane protein on B lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. CR2, together with CD19, CD81, and CD225, forms the B-cell coreceptor complex, which lowers the activation threshold of the B-cell antigen receptor. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9).
ID:P20023
ID:1380

alias

C3DR|CD 21|CD21|CD21 antigen|Complement C3d receptor|Complement component (3d/Epstein Barr virus) receptor 2|Complement component receptor 2|Complement receptor type 2|Cr 2|CR|Cr2|CR2_HUMAN|CVID7|EBV receptor|EBV-R|Epstein Barr virus receptor|Epstein-Barr virus receptor|EVBR|SLEB9

Origin

Nativ and recombinant human CD21

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

CHO, Ile21-71

sensitivity

<20pg/ml

detection range

0.78ng/ml-50ng/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|9.7|18.4|34.5|12.2|21.6|38.8
Standard deviation|0.44|0.77|1.24|0.77|1.23|1.86
CV(%)|4.5|4.2|3.6|6.3|5.7|4.8

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human CD21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD21 has been precoated onto 96-well plates. Standards(CHO, Ile21-71) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD21 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 50ng/ml, 25ng/ml, 12.5ng/ml, 6.25ng/ml, 3.12ng/ml, 1.56ng/ml, 0.78ng/ml human CD21 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human CD21 standard solution and each sample be measured in duplicate.

tipical result

Concentration(ng/ml)|0|0.78|1.56|3.12|6.25|12.5|25|50 O.D.|0.003|0.355|0.665|1.038|1.494|1.972|2.210|2.198

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human CD21 antibody|1
ST0000-50|Lyophilized recombinant human CD21 standard|50ng/tube�2
AR1107|Biotinylated anti- human CD21 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4