Human CD200 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human CD200 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of CD200 in Human serum, plasma and cell culture media. An antibody specific for Human CD200 was coated on a 96-well plate. Standards and samples are added to the wells and any CD200 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human CD200 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of CD200 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Virla infection;

specific against

Human

detection target

CD200 molecule(CD200)
Background: CD200 antigen, also known as OX-2, is a type I transmembrane protein belonging to the Ig superfamily. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. CD200 is widely expressed in multiple cell types. It interacts with CD200 R1, another Ig superfamily inhibitory receptor primarily expressed on leukocytes of the myeloid lineage, to inhibit myeloid functions. CD200 R1 is a transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed primarily in cells of the myeloid lineage, while its ligand, CD200 R1 is though to propagate inhibitory signals and disruption of the CD200/CD200 R1 receptor-ligand pair leads to a predisposition to autoimmune phenotypes.

alias

MOX1, MOX2, MRC, OX-2

sensitivity

18 pg/ml

detection range

24.6-6000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P41217