Human CD163 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human CD163 in cell culture supernates, serum and plasma(heparin).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human CD163
Protein name: Scavenger receptor cysteine-rich type 1 protein M130
Gene Full Name: CD163 molecule
abbreviation: CD163
Background: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.
ID:Q86VB7
ID:9332

alias

CD_antigen=CD163| CD163| Hemoglobin scavenger receptor| M130| MM130| Scavenger receptor cysteine-rich type 1 protein M130

Origin

Nativ and recombinant human CD163

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, G41-S1045

sensitivity

<150pg/ml

detection range

1.56ng/ml-100ng/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|24|38.1|65|23|37.2|67
Standard deviation|1.008|1.715|3.445|1.541|2.009|3.752
CV(%)|4.2|4.5|5.3|6.7|5.4|5.6

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human CD163 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD163 has been precoated onto 96-well plates. Standards(NSO, G41-S1045) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD163 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD163 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 100ng/ml?50ng/ml, 25ng/ml?12.5ng/ml?6.25ng/ml?3.13ng/ml?1.56ng/ml human CD163 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each human CD163 standard solution and each sample is measured in duplicate.

tipical result

Concentration(ng/ml)|0|1.56|3.12|6.25|12.5|25|50|100 O.D.|0.012|0.046|0.096|0.205|0.449|0.973|1.864|2.469

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human CD163 antibody|1
ST0000-100|Lyophilized recombinant human CD163 standard|100ng/tube�2
AR1107|Biotinylated anti- human CD163 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4