The human CD146 ELISA Pair Set is for the quantitative determination of human CD146. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD146(MCAM / MUC18) coated on a 96-well plate. Standards and samples are added tothe wells, and any CD146 present binds to the immobilized antibody. The wellsare washed and a horseradish peroxidase conjugated rabbit anti-CD146 polyclonalantibody is then added, producing an antibody-antigen-antibody "sandwich". Thewells are again washed and TMB substrate solution is loaded, which producescolor in proportion to the amount of CD146 present in the sample. To end theenzyme reaction, the stop solution is added and absorbances of the microwellare read at 450 nm.
MCAMStandard (antigene): Each vial contains 25 ng of recombinant CD146. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended. capture antibody type: 0.5 mg/mL of mouse anti-CD146 monoclonal antibody. Dilute to a working concentration of 2 µg/mL in CBS before coating. (Catalog: # 10115-MM05)detection antibody type: 0.5 mg/mL rabbit anti-CD146 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 µg/mL in detection antibody dilution buffer before use (Catalog: # 10115-R044)Lable: HRP
The minimum detectable dose of human CD146 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard..
The average concentration of CD146 in 36 normal human serum is 296.7+/-65.52 ng/mL ranging from 200.9 to 429.6 ng/mL.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of human CD146 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard..|
|Detection Range||31.25 pg/ml|
|Alias||CD146 / MCAM / MUC18|