For the quantitative determination of Human CD146 / MCAM concentration in serum.
The average concentration of Human CD146 detected in 36 human serum from apparently healthy people is 296.7 +/- 65.5ng/mL ranging from 200.9 to 429.6 ng/mL.
This assay recognizes both recombinant and natural Human CD146.The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
MCAMAntigene: 10115-H08Hcapture antibody type: mouse mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||31.25-2000 pg/ml|
|Alias||CD146 / MCAM|