Human CD13/Aminopeptidase N PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of Human CD13 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human CD13
Protein name: Aminopeptidase N
Gene Full Name: alanyl (membrane) aminopeptidase
abbreviation: ANPEP
Background: Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility.
ID:P15144
ID:290

alias

Alanyl (membrane) aminopeptidase|Alanyl aminopeptidase|Alanyl membrane aminopeptidase|Aminopeptidase M|Aminopeptidase N|AMPN_HUMAN|ANPEP|AP M|AP N|AP-M|AP-N|APN|CD 13|CD13|CD13 antigen|gp150|hAPN|Lap 1|Lap1|Microsomal aminopeptidase|Myeloid plasma membrane glycoprotein CD13|p150|PEPN

Origin

Nativ and recombinant human CD13

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, K69-K967

sensitivity

<10pg/ml

detection range

312pg/ml-20000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|2.98|6.54|12.73|3.27|6.83|13.76
Standard deviation|0.158|0.360|0.751|0.190|0.430|0.922
CV(%)|5.3|5.5|5.9|5.8|6.3|6.7

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human CD13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD13 has been precoated onto 96-well plates. Standards(NSO, K69-K967) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD13 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human CD13 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human CD13 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|312|625|1250|2500|5000|10000|20000 O.D.|0.027|0.122|0.183|0.336|0.584|1.098|1.807|2.273

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human CD13 antibody|1
ST0000-20|Lyophilized recombinant human CD13 standard|20ng/tube�2
AR1107|Biotinylated anti- human CD13 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4