Human CD10/Neprilysin PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human CD10 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human CD10
Protein name: Neprilysin
Gene Full Name: membrane metallo-endopeptidase
abbreviation: MME
Background: CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), Neprilysin, or common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.
ID:P08473
ID:4311

alias

Atriopeptidase|CALLA|CD 10|CD10|CD10 antigen|Common acute lymphocytic leukemia antigen|DKFZp686O16152|EC 3.4.24.11|Enkephalinase|EPN|gp100|Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase)|Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10)|Membrane metallo endopeptidase|Membrane metallo endopeptidase variant 1|Membrane metallo endopeptidase variant 2|Membrane metalloendopeptidase|Membrane metalloendopeptidase neutral endopeptidase enkephalinase|Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10|Membrane metalloendopeptidase variant 1|Membrane metalloendopeptidase variant 2|MGC126681|MGC126707|MME|NEP|NEP_HUMAN|Neprilysin|Neutral endopeptidase 24.11|Neutral endopeptidase|SFE|Skin fibroblast elastase

Origin

Nativ and recombinant human CD10

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

CHO, Y52-W750

sensitivity

<10pg/ml

detection range

125pg/ml-8000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1265|3210|6840|1526|2761|6397
Standard deviation|48.07|179.8|321.5|83.93|201|369.6
CV(%)|3.8|5.6|4.7|5.5|7.3|6.2

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human CD10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD10 has been precoated onto 96-well plates. Standards(CHO, Y52-W750) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD10 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml human CD10 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human CD10 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|125|250|500|1000|2000|4000|8000 O.D.|0.097|0.148|0.196|0.319|0.462|0.858|1.328|1.965

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human CD10 antibody|1
ST0000-10|Lyophilized recombinant human CD10 standard|10ng/tube�2
AR1107|Biotinylated anti- human CD10 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4