The Human Carbonic Anhydrase XII / CA12 ELISA Pair Set is for the quantitative determination of Human Carbonic Anhydrase XII / CA12. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Carbonic Anhydrase XII / CA12 coated on a 96-well plate. Standards and samples are added to the wells, and any Carbonic Anhydrase XII / CA12 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Carbonic Anhydrase XII / CA12 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Carbonic Anhydrase XII / CA12 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
CA12Standard (antigene): Each vial contains 37 ng of recombinant CA12. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.5 ng/mL is recommended. capture antibody type: 90 µg/mL of mouse anti-CA12 monoclonal antibody, Dilute to a working concentration of 2 µg/mL in CBS before coating. (Catalog: # 10617-MM01)detection antibody type: 0.25 mg/mL mouse anti-CA12 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 µg/mL in detection antibody dilution buffer before use. (Catalog: # 10617-MM08)Lable: HRP
The minimum detectable dose of Human Carbonic Anhydrase XII / CA12 was determined to be approximately 7.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of Human Carbonic Anhydrase XII / CA12 was determined to be approximately 7.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|Antigen Name||Carbonic Anhydrase XII|
|Detection Range||7.8 pg/ml|
|Alias||Carbonic Anhydrase XII / CA12|