Human C5a ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human C5a ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of C5a in Human serum, plasma and cell culture media. An antibody specific for Human C5a was coated on a 96-well plate. Standards and samples are added to the wells and any C5a present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human C5a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of C5a present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Coagulation ;

specific against

Human

detection target

Complement C5(C5a)
Background: C5a is a protein fragment released from cleavage of complement component C5 by protease C5-convertase into C5a and C5b fragments. C5b is important in late events of the complement cascade, an orderly series of reactions which coordinates several basic defense mechanisms, including formation of the Membrane Attack Complex, one of the most basic weapons of the innate immune system, formed as an automatic response to intrusions from foreign particles and microbial invaders. It essentially pokes microscopic pinholes in these foreign objects, causing loss of water and sometimes death. C5a acts as a highly inflammatory peptide, encouraging complement activation, formation of the MAC, attraction of immune cells, and histamine release involved in allergic responses. C5a is a chemotactic agent and an anaphylatoxin; it is essential in the innate immunity but it is also linked with the adaptive immunity. The increased production of C5a is connected with a number of inflammatory diseases.

alias

C5b, C5a, CPAMD4

sensitivity

20 pg/ml

detection range

31.2-2000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P01031