Human beta-NGF EasyTestTM ELISA kit

Brand

BioAim Scientific (Kanada)

short description

The BioAim Human Beta-NGF EasyTestTM ELISA kit can quantitatively measure Beta-NGF in human serum or plasma. It is a simple and rapid technology for the quantitation of antigen in a range of sample matrices. The whole process takes less than 1.5 hours with high accuracy and precision. EasyTestTM ELISA is faster and easier to perform than standard format ELISA with less reagent handling and fewer pipetting steps.
Research Area Apoptosis, Cell growth and differentiation;

specific against

Human

detection target

nerve growth factor beta polypeptide(β-NGF)
Background: Nerve growth factor (NGF) was initially isolated in the mouse submandibular gland over three decades ago as a 7S complex composed of three non- covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases, while the beta subunit, called beta-NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. As its name suggests, NGF is involved primarily in the growth, as well as the maintenance, proliferation, and survival of nerve cells (neurons). In fact, NGF is critical for the survival and maintenance of sympathetic and sensory neurons, as they undergo apoptosis in its absence. However, several recent studies suggest that NGF is also involved in pathways besides those regulating the life cycle of neurons. NGF binds with at least two classes of receptors: the tropomyosine receptor kinase A (TrkA) and low-affinity NGF receptor (LNGFR/p75NTR). Both are associated with neurodegenerative disorders. When NGF binds to the TrkA receptor, it drives the homodimerization of the receptor, which in turn causes the autophosphorylation of the tyrosine kinase segment. This leads to the activation of PI 3-kinase, ras, and PLC signaling pathways. Alternatively, the p75NTR receptor can form a heterodimer with TrkA, which has higher affinity and specificity for NGF.

alias

Beta-NGF, HSAN5, NGFB

sensitivity

<1 pg/ml

detection range

31.2-2000 pg/ml

sample type

Serum

pack size

1x96 Wells

Assay time

1.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P01138