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Human beta-IGH3 ELISA Kit

Product Code: BAS-3010036

Verfügbarkeit: Auf Lager

Human beta-IGH3 ELISA Kit

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Beschreibung

Details

Human beta-IGH3 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human beta-IGH3 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of beta-IGH3 in human serum and plasma. An antibody specific for human beta-IGH3 was coated on a 96-well plate. Standards and samples are added to the wells and any beta-IGH3 present binds to the immobilized antibody. The wells are washed and biotinylated anti-human beta-IGH3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of beta-IGH3 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis;

specific against

Human

detection target

transforming growth factor beta induced (β-IGH3 )
Background: Beta IG-H3, also known as TGFBI and RGD-CAP, is a matricellular adaptor protein that is induced in most cell types in response to TGF-beta stimulation .Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a proteinwhich in humans is encoded by the TGFBI gene, locus 5q31. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in manyextracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations of the gene cause several forms of corneal dystrophies.

alias

CDGG1, LCD1, CSD2, CSD, CSD1, BIGH3, CDG2, CSD3, CDB1, EBMD

sensitivity

10 pg/ml

detection range

24.6-6000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q15582

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller BioAim Scientific
Herstellerland Kanada
Sensitivity 10 pg/ml
Specific Against Human
Antigen Name transforming growth factor beta induced 
Detection Range 24.6-6000 pg/ml
Alias CDGG1, LCD1, CSD2, CSD, CSD1, BIGH3, CDG2, CSD3, CDB1, EBMD
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