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BioAim Scientific (Kanada)
The Bioaim Human BCAM ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of BCAM in Human serum, plasma and cell culture media. An antibody specific for Human BCAM was coated on a 96-well plate. Standards and samples are added to the wells and any BCAM present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human BCAM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of BCAM present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Angiogenesis; cell adhesion; inflammation;
Human
Basal cell adhesion molecule(BCAM) Background: Basal cell adhesion molecule (BCAM) and Lutheran blood group glycoprotein (LU) are two alternatively spliced variants of a single immunoglobulin superfamily protein that differ in the length of their cytoplasmic tails. Lutheran blood group glycoprotein is a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five, N-terminus, extracellular immunoglobulin domains, a single transmembrane domain, and a short, C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.
AU, CD239, LU, MSK19
20 pg/ml
40.9-10000 pg/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
P50895
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | 20 pg/ml |
Specific Against | Human |
Antigen Name | Basal cell adhesion molecule |
Detection Range | 40.9-10000 pg/ml |
Alias | AU, CD239, LU, MSK19 |