For the quantitative determination of Human B7-H3 / CD276 concentration in serum and urine.
The average concentration of Human B7H3 in 10 normal human serum is 2188.44 +/- 692.04 pg/mL ranging from 1365.12 to 3598.60 pg/mL.The average concentration of Human B7H3 in 6 normal human urine is 390.20 +/- 268.93 pg/mL ranging from 83.33 to 678.93 pg/mL.
This assay recognizes both recombinant and natural Human B7H3. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
CD276Antigene: 11188-H08Hcapture antibody type: mouse mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||23.44-1500 pg/ml|
|Alias||B7-H3 / CD276|