For the quantitative determination of Human ASGPR1 concentration in serum and cell culture supernates.
The average concentration of Human ASGR1 in 8 normal human serum is 189.39 +/- 65.77 pg/mL ranging from 101.57 to 274.92 pg/mL. HepG2 cells (0.2 x 10E5 cells/mL) were cultured 3 days. An aliquot of the cell culture supernate was removed, assayed for levels of natural ASGR1, and measured 424.39 pg/mL.
This assay recognizes both recombinant and natural Human ASGR1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
ASGR1Antigene: 10773-H07Hcapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||31.25-2000 pg/ml|