Human Apoptosis Signaling Pathway Array C1

Brand

Raybiotech

short description

RayBio® C-Series Human Apoptosis Signaling Pathway Array C1. Detects 19 phosphorylated or cleaved human Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Intended Use Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process;

specific against

Human

detection target

Akt (P-Ser473) (PKB (P-Ser473)), ATM (P-Ser1981), BAD (P-Ser112), Caspase-3 (Cleaved Asp175), Caspase-7 (Cleaved Asp198), CHK1 (P-Ser296), CHK2 (P-Thr68), eIF-2a (P-Ser52), ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187), HSP27 (P-Ser82), IKBa (P-Ser32), JNK (P-Thr183/Tyr185), NFKBP65 (P-Ser536), PARP1 (Cleaved Asp214/Gly215), p27 (P-Thr198), P53 (P-Ser15), SMAD2 (Ser245/250/255), TAK1 (P-Ser412)

certificate

GMP/ISO

pack size

2, 4 and 8 Sample Kit

storage and Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Principle of the Elisa

Apoptosis Arrays - Sandwich-based

Kit Content

• Human Apoptosis Signaling Pathway Phosphorylation Antibody Array C1 Membranes
• Blocking Buffer
• Wash Buffer 1
• Wash Buffer 2
• Detection Antibody Cocktail
• HRP-Conjugated Anti-IgG
• Detection Buffer C
• Detection Buffer D
• Lysis Buffer
• Protease Inhibitor Cocktail
• Phosphatase Inhibitor Cocktail Set I
• Phosphatase Inhibitor Cocktail II
• 8-Well Incubation Tray
• Plastic Sheets
• Array Templates
• Manual