BioAim Scientific (Kanada)
The Bioaim Human ALCAM ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of ALCAM in Human serum, plasma and cell culture media. An antibody specific for Human ALCAM was coated on a 96-well plate. Standards and samples are added to the wells and any ALCAM present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human ALCAM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of ALCAM present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Angiogenesis; cell adhesion; inflammation;
activated leukocyte cell adhesion molecule(ALCAM) Background: Activated leukocyte cell adhesion molecule (ALCAM) is a typeI membrane glycoprotein that is a member of the immunoglobulin superfamily. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. In humans, it is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development.
Serum, plasma, Cell culture supernatant
|Antigen Name||activated leukocyte cell adhesion molecule|
|Detection Range||20.5-5000 pg/ml|