Human ALCAM ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human ALCAM ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of ALCAM in Human serum, plasma and cell culture media. An antibody specific for Human ALCAM was coated on a 96-well plate. Standards and samples are added to the wells and any ALCAM present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human ALCAM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of ALCAM present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; cell adhesion; inflammation;

specific against

Human

detection target

activated leukocyte cell adhesion molecule(ALCAM)
Background: Activated leukocyte cell adhesion molecule (ALCAM) is a typeI membrane glycoprotein that is a member of the immunoglobulin superfamily. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. In humans, it is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development.

alias

CD166, MEMD

sensitivity

10 pg/ml

detection range

20.5-5000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q13740