The Human AFP / alpha-fetoprotein ELISA Pair Set is for the quantitative determination of Human AFP / alpha-fetoprotein. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for AFP / alpha-fetoprotein coated on a 96-well plate. Standards and samples are added to the wells, and any AFP / alpha-fetoprotein present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-AFP / alpha-fetoprotein monoclonal antibody is then added, producing an antibody-antigen-antibody ﾓsandwichﾔ. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of AFP / alpha-fetoprotein present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
AFPStandard (antigene): Each vial contains 38 ng of recombinant AFP. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.7 ng/mL is recommended. capture antibody type: 0.1 mg/mL of mouse anti-AFP monoclonal antibody. Dilute to a working concentration of 2 µg/mL in CBS before coating. (Catalog: # 12177-MM33)detection antibody type: 0.5 mg/mL mouse anti-AFP monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 µg/mL in detection antibody dilution buffer before use. (Catalog: # 12177-MM26)Lable: HRP
The minimum detectable dose of Human AFP / alpha-fetoprotein was determined to be approximately 11 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
The average concentration of AFP in 10 normal human serum is 2.34+/-0.95 ng/mL ranging from 0.97 to 3.81 ng/mL.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of Human AFP / alpha-fetoprotein was determined to be approximately 11 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|Detection Range||11 pg/ml|
|Alias||AFP / alpha-fetoprotein|