×

Registrierung

Profile Informationen

Login Daten

oder Einloggen

Vorname ist erforderlich!
Nachname ist erforderlich!
Vorname ist ungültig!
Nachname ist ungültig!
Dies ist keine E-Mail-Adresse!
E-Mail-Adresse ist erforderlich!
Dies E-Mail-Adresse ist bereit registriert!
Passwort ist erforderlich!
Geben Sie ein gültiges Passwort ein!
Bitte geben Sie 6 oder mehr Zeichen ein!
Bitte geben Sie 16 oder weniger Zeichen ein!
Passwörter sind nicht dasselbe!
Geschäftsbedingungen sind erforderlich!
E-Mail oder Passwort ist falsch!

Human aFGF ELISA Kit

Product Code: BAS-3010151

Verfügbarkeit: Auf Lager

Human aFGF ELISA Kit

Bewerten Sie dieses Produkt als Erster

OR

Fragen zur Lieferzeit

Angebot anfordern

Beschreibung

Details

Human aFGF ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human aFGF ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of aFGF in Human serum, plasma and cell culture media. An antibody specific for Human aFGF was coated on a 96-well plate. Standards and samples are added to the wells and any aFGF present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human aFGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of aFGF present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; cell growth; Cancer Biomarker;

specific against

Human

detection target

Fibroblast growth factor 1(aFGF)
Background: FGF acidic, also known as FGF-1, ECGF, and HBGF-1, is a secreted mitogen that stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF acidic is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Internalized FGF acidic can translocate to the cytosol and to the nucleus. Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.

alias

AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-1, FGF-alpha, FGFA, GLIO703, HBGF-1, HBGF1

sensitivity

10 pg/ml

detection range

16.4-4000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P05230

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller BioAim Scientific
Herstellerland Kanada
Sensitivity 10 pg/ml
Specific Against Human
Antigen Name Fibroblast growth factor 1
Detection Range 16.4-4000 pg/ml
Alias AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-1, FGF-alpha, FGFA, GLIO703, HBGF-1, HBGF1
Bewertungen
Tags

Produkttags

Verwenden Sie Leerzeichen um Tags zu trennen. Verwenden Sie Apostrophe (') für Phrasen.

Datasheet

Video