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BioAim Scientific (Kanada)
The Bioaim Human Activin A ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Activin A in human serum and plasma. An antibody specific for human Activin A was coated on a 96-well plate. Standards and samples are added to the wells and any Activin A present binds to the immobilized antibody. The wells are washed and biotinylated anti-human Activin A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Activin A present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area neural cell differentiation, bone remodeling, hematopoiesis;
Human
inhibin, beta A(Activin A ) Background: Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. activin enhances FSH biosynthesis and secretion, and participates in the regulation of the menstrual cycle. Many other functions have been found to be exerted by activin, including roles in cell proliferation, differentiation, apoptosis, metabolism, homeostasis, immune response, wound repair, and endocrine function. Conversely, inhibin downregulates FSH synthesis and inhibits FSH secretion. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits (mammalian beta A, beta B, beta C, beta E and Xenopus beta D) have been cloned to date. The activin/inhibin nomenclature reflects the subunit composition of the proteins: Activin A (beta A – beta A), Activin B (beta B – beta B), Activin AB (beta A – beta B), Inhibin A (alpha – beta A) and Inhibin B (alpha – beta B).
FRP, EDF
10 pg/ml
20.5-5000 pg/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
P08476
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | 10 pg/ml |
Specific Against | Human |
Antigen Name | inhibin, beta A |
Detection Range | 20.5-5000 pg/ml |
Alias | FRP, EDF |