Glycan Array 100

Brand

Raybiotech

short description

Detection of binding partners for 100 different glycans in purified proteins, serum, plasma, cell culture supernatants, cell/tissue lysates or other body fluids.
Intended Use

• Identify the glycans binding partners in biological samples
• Identify whether target proteins are carbohydrate binding proteins
• Probe binding of viruses and whole cells to glycans^1-3
• Profile the substrate specificity of enzymes (glycosyltransferases, glycosidases, etc.)^4-6
• Profile the inflammatory immune response

detection target

?-Glc-, ?-Gal-, ?-Man-, ?-Fuc-, ?-Rha-, ?-GlcNAc-, ?-GalNAc-, Tobramycin, Gal-?-1,3-GlcNAc-?-, Gal-?-1,3-Gal-?-1,3-GlcNAc-?-, Neu5Ac-?-2,3-Gal-?-1,3-GlcNAc-?-, Neu5Ac-?-2,6-Gal-?-1,3-GlcNAc-?-, Neu5Gc-?-2,3-Gal-?-1,3-GlcNAc-?-, Neu5Gc-?-2,6-Gal-?-1,3-GlcNAc-?-, Gal-?-1,3-(Fuc-?-1,4)-GlcNAc-?- [Lewis A], Gal-?-1,4-Glc-?-, Gal-?-1,3-Gal-?-1,4-Glc-?-, Gal-?-1,4-Gal-?-1,4-Glc-?-, GlcNAc-?-1,3-Gal-?-1,4-Glc-?-, GalNAc-?-1,3-Gal-?-1,4-Glc-?-, Neu5Ac-?-2,3-Gal-?-1,4-Glc-?-, Neu5Ac-?-2,6-Gal-?-1,4-Glc-?-, Neu5Gc-?-2,3-Gal-?-1,4-Glc-?-, Neu5Ac-?-2,6-Gal-?-1,4-Glc-?-, Gal-?-1,4-(Fuc-?-1,3)-Glc-?-, GalNAc-?-1,3-Gal-?-1,4-Gal-?-1,4-Glc-?-, GlcNAc-?-1,6-GlcNAc-?-, 4-P-GlcNAc-?-1,4-Man-?-, Glc-?-1,2-Gal-?-1,3-Glc-?-, Gal-?-1,3-GalNAc-?-, Gal-?-1,4-GlcNAc-?-, Gal-?-1,4 -(Fuc-?-1,3)-GlcNAc-?- [Lewis X], Neu5Ac-?-2,3-Gal-?-1,4-(Fuc-?-1,3)-GlcNAc-?- [Sialyl Lewis X], Neu5Ac-?-2,3-Gal-?-1,3 -(Fuc-?-1,4)-GlcNAc-?- [Sialyl Lewis A], Neu5Gc-?-2,3-Gal-?-1,3-(Fuc-?-1,4)-GlcNAc-?- [Sialyl Lewis A], Gal-?-1,4-Gal-?-1,3-GlcNAc-?-, Gal-?-1,4-GlcNAc-?-1,3-Gal-?-1,4-Glc-?- [LNnT], GlcA-?-1,4-GlcNAc-?-1,4-GlcA-?-, GlcNAc-?-1,6-(Gal-?-1,3)-GalNAc-?-O-Ser, Neu5Ac-?-2,3Gal-?-1,4-(6S)GlcNAc-?-, GalNAc-?-1,4-GlcNAc-?-, Neu5Ac-?-2,8-Neu5Ac-?-2,3-Gal ?-1,4-Glc-?-, Neu5Gc-?-2,8-Neu5Ac-?-2,3-Gal-?-1,4-Glc-?-, GalNAc-?-1,3-(Fuc-?-1,2)-Gal-?-1,4-Glc-?- [Blood A antigen tetrose], GlcNAc-?-1,2-Man-?-, Neu5Ac-?-2,3-Gal-?-, Gal-?-1,3 -GalNAc-?-1,3-Gal-?-, Glc-?-1,2-Gal-?-, Gal-?-1,4-(Fuc-?-1,3)-GlcNAc-?-1,3-Gal-?-, Neu5Ac-?-2,3-Gal-?-1,4-(Fuc-?-1,3)-Glc-?- [3-Sialyl-3-fucosyllactose/ F-SL], GlcNAc-?-1,4-GlcNAc-?-, ?-D-GlcA-, Gal-?-1,4-(6S)GlNAc-?-, GlcNAc-?-1,3-(Glc-?-1,2-Glc-?-1,2)-Gal-?-1,3-Glc-?-, Gal-?-1,3-GalNAc-?-1,4-(Neu5Gc-?-2,3)-Gal-?-1,4-Glc-?-, Sisomicin Sulfate, GalNAc-?-1,3-(Fuc-?-1,2)-Gal-?- [Blood A antigen trisaccharide], Fuc-?-1,2-Gal-?-1,4-GlcNAc-?- [Blood H antigen trisaccharide], Gal-?-1,3-(Fuc-?-1,2)-Gal-?- [Blood B antigen trisaccharide], Fuc-?-1,2-Gal-?-1,3-GlcNAc-?-1,3-Gal-?-1,4-Glc-?- [LNFP I], Fuc-?-1,2-Gal-?-1,4-Glc-?- [Blood H antigen trisaccharide], Gal-?-1,3-(Fuc-?-1,2)-Gal-?-1,4-Glc-?- [Blood B antigen tetrasaccharide], (Fuc-?-1,2)-Gal-?-1,4-(Fuc-?-1,3)-GlcNAc-?- [ Lewis Y], (Fuc-?-1,2)-Gal-?-1,3-(Fuc-?-1,4)-GlcNAc-?- [ Lewis B], Gal-?-1,3-(Fuc-?-1,4)-GlcNAc-?-1,3-Gal-?-1,4-(Fuc-?-1,4)-Glc-?- [Lewis A], Gal-?-1,3-GalNAc-?-, Gal-?-1,3-(Neu5Ac-?-2,6)-GalNAc-?-, Neu5Ac-?-2,6-Gal-?-1,3-GalNAc-?-, Neu5Ac-?-2,6-Gal-?-1,3-(Neu5Ac-?-2,6)-GalNAc-?-, Neu5Ac-?-2,3-Gal-?-1,3-(Neu5Ac-?-2,6)-GalNAc-?-, Neu5Ac-?-2,6-(Neu5Ac-?-2,3)-Gal-?-1,3-GalNAc-?-, GalNAc-?-1,4-(Neu5Ac-?-2,3)-Gal-?-1,4-Glc-?- [GM2], GalNAc-?-1,4-(Neu5Ac-?-2,8-Neu5Ac-?-2,3)-Gal-?-1,4-Glc-?- [GD2], Gal-?-1,4-Gal-?-1,4-GlcNAc-?-, ?-D-Rha-, Glc-?-1,4-Glc-?-, Glc-?-1,6-Glc-?-1,4-Glc-?-, Maltotriose-?-, Glc-?-1,6-Glc-?-1,6-Glc-?-, Maltotetraose-?-, GlcNAc-?-1,4-GlcA-?-1,4-GlcNAc-?1,4-GlcA-?-, Maltohexaose-?-, Maltoheptaose-?-, Acarbose-?-, D-pentamannuronic acid-?-, L-pentaguluronic acid-?-, D-cellose-?-, Gal-?-1,3-Gal-?-, ?-1,4-Xylotetrose, Chitin-trisaccharide, KDN-?-2,8-Neu5Ac-?-2,3-Gal-?-1,4-Glc-?-, Neu5Ac-?-2,8-Neu5Gc-?-2,3-Gal-?-1,4-Glc-?-, Neu5Ac-?-2,8-Neu5Ac-?-2,8-Neu5Ac-?-2,3-Gal-?-1,4-Glc-?-, Neu5Ac-?-2,8-Neu5Ac-?-2,6-Gal-?-1,4-Glc-, Gal-?-1,3-GalNAc-?-1,4-(Neu5Ac-?-2,3)-Gal-?-1,4-Glc-?-, Gentamicin Sulfate, Kanamycin sulfate, Geneticin Disulfate Salt (G418), Neomycin trisulfate, SGP

certificate

GMP/ISO

pack size

4, 8 and 16 Sample Kit

storage and Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Principle of the Elisa

Glycan Arrays - Label-based; Sandwich-based

Kit Content

• Dialysis Vials
• Labeling Reagent
• Stop Solution
• Glycan Array Glass Slide Assembly
• Sample Diluent
• 20X Wash Buffer I
• 20X Wash Buffer II
• Cy3 equivalent dye-conjugated Streptavidin
• Slide Washer/Dryer
• Adhesive device sealer
• Labeling Buffer
• Floating Dialysis Rack