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ELISA Kit for Interleukin 11 Receptor Alpha (IL11Ra) - SEE771Mu

Product Code: SEE771Mu

Verfügbarkeit: Auf Lager

ELISA Kit for Interleukin 11 Receptor Alpha (IL11Ra) - SEE771Mu

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Beschreibung

Details

ELISA Kit for Interleukin 11 Receptor Alpha (IL11Ra)

Brand

Cloud-Clone

short description

Enzyme-linked immunosorbent assay for Antigen Detection.
Research focus: Infection immunity; ;

spezie

Mus musculus (Mouse)

Cross Reaction

This assay has high sensitivity and excellent specificity for detection of Interleukin 11 Receptor Alpha (IL11Ra).
No significant cross-reactivity or interference between Interleukin 11 Receptor Alpha (IL11Ra) and analogues was observed.

detection target

Interleukin 11 Receptor Alpha

alias

sensitivity

The minimum detectable dose of this kit is typically less than 0.061 ng/mL

detection range

0.156-10 ng/mL

certificate

ISO 9001:2008, ISO 13485:2003

sample type

Tissue homogenates and other biological fluids.

pack size

48T, 96T, 5x96T, 10x96T

Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 11 Receptor Alpha (IL11Ra) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 11 Receptor Alpha (IL11Ra) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Assay time

3h

storage and Stability

All the kits should stored according to the manual.
The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree upon receipt while the others should be at 4 degree.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Principle of the Elisa

Double-antibody Sandwich
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 11 Receptor Alpha (IL11Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 11 Receptor Alpha (IL11Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 11 Receptor Alpha (IL11Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 11 Receptor Alpha (IL11Ra) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Kit Content

Pre-coated, ready to use 96-well strip plate: 1
Plate sealer for 96 wells: 4
Standard: 2
Standard Diluent: 1x20mL
Detection Reagent A: 1x120µ L
Assay Diluent A: 1x12mL
Detection Reagent B: 1x120µ L
Assay Diluent B: 1x12mL
TMB Substrate: 1x9mL
Stop Solution: 1x6mL
Wash Buffer (30 x concentrate): 1x20mL
Instruction manual: 1

Assay procrdure summery

1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.

Uniport ID

Q64385

Weitere Informationen

Weitere Informationen

Hersteller Cloud-Clone
Herstellerland China
Sensitivity The minimum detectable dose of this kit is typically less than 0.061 ng/mL
Specific Against Mouse
Antigen Name Interleukin 11 Receptor Alpha (IL11Ra)
Detection Range 0.156-10 ng/mL
Zertifikat ISO 9001:2008, ISO 13485:2003
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