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Enzyme-linked immunosorbent assay for Antigen Detection.Research focus: CD & Adhesion molecule; Tumor immunity; Infection immunity; Immune molecule; ;
Mus musculus (Mouse)
This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 14 (CD14).
No significant cross-reactivity or interference between Cluster Of Differentiation 14 (CD14) and analogues was observed.
Cluster Of Differentiation 14
Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein
The minimum detectable dose of this kit is typically less than 0.138 ng/mL
0.312-20 ng/mL
ISO 9001:2008, ISO 13485:2003
Serum, plasma, tissue homogenates and other biological fluids.
48T, 96T, 5x96T, 10x96T
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 14 (CD14) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 14 (CD14) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
3h
All the kits should stored according to the manual. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree upon receipt while the others should be at 4 degree.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Double-antibody SandwichTest principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 14 (CD14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 14 (CD14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 14 (CD14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 14 (CD14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Pre-coated, ready to use 96-well strip plate: 1Plate sealer for 96 wells: 4Standard: 2 Standard Diluent: 1x20mL Detection Reagent A: 1x120µ L Assay Diluent A: 1x12mL Detection Reagent B: 1x120µ L Assay Diluent B: 1x12mL TMB Substrate: 1x9mL Stop Solution: 1x6mL Wash Buffer (30 x concentrate): 1x20mL Instruction manual: 1
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.
Hersteller | Cloud-Clone |
---|---|
Herstellerland | China |
Sensitivity | The minimum detectable dose of this kit is typically less than 0.138 ng/mL |
Specific Against | Mouse |
Antigen Name | Cluster Of Differentiation 14 (CD14) |
Detection Range | 0.312-20 ng/mL |
Zertifikat | ISO 9001:2008, ISO 13485:2003 |
Referenz | Successful Treatment of Acute Lung Injury with Pitavastatin in Septic Mice: Potential Role of Glucocorticoid Receptor Expression in Alveolar Macrophages; |
Alias | Monocyte differentiation antigen CD14/ Myeloid cell-specific leucine-rich glycoprotein |