CytoTracker Solution and TNFa should be removed from the kit and stored at -20C immediately. Store all other components at 4C.
Cancer cell transmigration, particularly extravasation, is an important step in cancer metastasis. it is the final step in a cascade of interactions between cells and the endothelium. CytoSelect Tumor Transendothelial Migration Assay provides a robust system for the quantitative determination of transmigrations and interactions between endothelium and tumor cells. Migratory cells may be quantified on a fluorescence plate reader. Kits use 24-well plates with 8 um pore size membrane inserts.
1. Quantify interactions between endothelium and tumor cells
2. Fully quantify cell transmigration with no manual cell counting
3. Highly sensitive results on a fluorescence plate reader