Store the CytoSelect Methylcellulose Medium at -20C. if the methylcellulose medium will not be used all at once, it should be aliquoted to avoid multiple freeze/thaw cycles. Store all other components at 4C.
Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.
1. Methylcellulose medium supports growth of hematopoietic cell colonies
2. Fully quantify hematopoietic stem cells with no manual cell counting
3. Results in 7-10 days, not 3 weeks