Shipping Temp:

Blue ice

Storage Temp:

Store the CytoSelect Methylcellulose Medium at -20C. if the methylcellulose medium will not be used all at once, it should be aliquoted to avoid multiple freeze/thaw cycles. Store all other components at 4C.

Description:

Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.

Formulation:

1. Methylcellulose medium supports growth of hematopoietic cell colonies

2. Fully quantify hematopoietic stem cells with no manual cell counting

3. Results in 7-10 days, not 3 weeks